Arbuscular Mycorrhiza Fungi Strengthen the Beneficial Effects of Warming on the Growth of Gynaephora Menyuanensis Larvae


Climate warming, Fungicide benomyl, AMF, Gynaephora menyuanensis.

How to Cite

Kelu Chen, Guoxi Shi, Wenjing Chen, Dangjun Wang, Wenying Wang, Zhen Ma, Buqing Yao, Huakun Zhou. Arbuscular Mycorrhiza Fungi Strengthen the Beneficial Effects of Warming on the Growth of Gynaephora Menyuanensis Larvae. Glob. J. Agric. Innov. Res. Dev [Internet]. 2018 Dec. 27 [cited 2023 May 31];5:24-8. Available from:


 Herbivores experience an unprecedented variously impacts of climate warming. Besides, arbuscular mycorrhiza fungi (AMF) also is influence on herbivores through their common host plants. Surprisingly, there are no reports about how AMF affect the responses of herbivores to warming. To close this gap, we conducted a two factors experiment to research the effects of warming, fungicide (AMF suppression), and their interaction on the development of Gynaephora menyuanensis larvae, an endemic generalist herbivore species in northeastern Tibetan Plateau, and nitrogen content of Elymus nutans, which was the main food of G. menyuanensis. Warming significantly advanced the pupation time (PT), expanded the phenomena of protandry and increased the growth rate (GR) of G. menyuanensis larvae. Fungicide not affected the development of G. menyuanensis larvae, despite their negative effects on the content of E. nutans. Warming with fungicide decreased the GR of G. menyuanensis compared with warming treatment. In other words, AMF strengthen the beneficial effects of warming to G. menyuanensis. This study provides the first evidence of the impacts of AMF on the response of herbivore to warming.


Shaver GR, Canadell J, Iii FSC, Gurevitch J, Harte J, Henry G, et al. Global warming and terrestrial ecosystems: A conceptual framework for analysis. Bioscience 2000: 50: 871-882.[0871:GWATEA

O'Connor MI. Warming strengthens an herbivore-plant interaction. Ecology 2009: 90: 388-398.

Durak R, Węgrzyn E & Leniowski K. Do all aphids benefit from climate warming? An effect of temperature increase on a native species of temperate climatic zone Cinara juniperi. Ethology Ecology & Evolution 2016: 28: 188-201.

Lemoine NP, Burkepile DE & Parker JD. Variable effects of temperature on insect herbivory. Peerj 2014: 2: e376-e376.

Megali L, Glauser G & Rasmann S. Fertilization with beneficial microorganisms decreases tomato defenses against insect pests. Agronomy for Sustainable Development 2014: 34: 649-656.

Kutyniok M & Müller C. Plant-mediated interactions between shoot-feeding aphids and root-feeding nematodes depend on nitrate fertilization. Oecologia 2013: 173: 1367-1377.

Zhu HUI, Wang D, Wang L, Fang J, Sun WEI & Ren B. Effects of altered precipitation on insect community composition and structure in a meadow steppe. Ecological Entomology 2014: 39: 453-461.

Barton, BT. Reduced wind strengthens top-down control of an insect herbivore. Ecology 2014; 95: 2375-2381.

Huang W, Siemann E, Carrillo J & Ding J. Below-ground herbivory limits induction of extrafloral nectar by aboveground herbivores. Annals of Botany 2015: 115: 841-846.

Hopper JV & Mills NJ. Novel multitrophic interactions among an exotic, generalist herbivore, its host plants and resident enemies in California. Oecologia 2016; 182: 1117-1128.

Tomczak VV, Schweiger R & Müller C. Effects of Arbuscular Mycorrhiza on Plant Chemistry and the Development and Behavior of a Generalist Herbivore. Journal of Chemical Ecology 2016; 1-12.

Minton MM, Barber NA & Gordon LL. Effects of arbuscular mycorrhizal fungi on herbivory defense in two Solanum (Solanaceae) species. Plant Ecology and Evolution 2016; 149: 157-164.

Kula A & Hartnett D. Effects of mycorrhizal symbiosis on aboveground arthropod herbivory in tallgrass prairie: an in situ experiment. Plant Ecology 2015: 216: 589-597.

Wang B & Qiu YL. Phylogenetic distribution and evolution of mycorrhizas in land plants. Mycorrhiza 2006; 16: 299-363.

Fitter A, Helgason T & Hodge A. Nutritional exchanges in the arbuscular mycorrhizal symbiosis: implications for sustainable agriculture. Fungal Biology Reviews 2011; 25: 68-72.

Balestrini R. Biological Potential of Arbuscular Mycorrhizal Fungi. In Bioformulations: for Sustainable Agriculture 2016 (pp. 127-135, Springer).

Borowicz VA. The impact of arbuscular mycorrhizal fungi on plant growth following herbivory: A search for pattern. Acta Oecologica 2013; 52: 1-9.

Koricheva J, Gange AC & Jones T. Effects of mycorrhizal fungi on insect herbivores: a meta-analysis. Ecology 2009; 90: 2088-2097.

Chen H, Zhu Q, Peng C, Wu N, Wang Y, Fang X, Gao Y et al. The impacts of climate change and human activities on biogeochemical cycles on the Qinghai-Tibetan Plateau. Global Change Biology 2013; 19: 2940-2955.

Gullan PJ. The insects: an outline of entomology. Journal of Insect Conservation 2015; 39: 231-232.

Wang YP, Wu H & Xu HC. Biological and ecological bases of using insect as a bio-indicator to asses forest health. Chinese Journal of Applied Ecology 2008; 19: 1625-1630.

Zhao X & Zhou X. Ecological Basis of Alpine Meadow Ecosystem Management in Tibet: Haibei Alpine Meadow Ecosystem Research Station. Ambio 1999; 28: 642-647.

Yu XC, Chen KL, Yao BQ, Ma Z, Wang WY, Wang HC et al. Effects of simulated warming on the growth and development of Gynaephora menyuanensis larvae. Acta Ecologica Sinica 2016; 36: 8002-8007.

Hartnett DC & Wilson GWT. The role of mycorrhizas in plant community structure and dynamics: lessons from grasslands. Plant & Soil 2002; 244: 319-331.

Smith MD, Hartnett DC & Rice CW. Effects of long-term fungicide applications on microbial properties in tallgrass prairie soil. Soil Biology & Biochemistry 2000; 32: 935-946.

Liu R & Chen Y. Mycorrhizology. China Science, Beijing 2007.

Cao H, Zhu WY & Zhao XQ. Effects of warming and grazing on growth and development of the grassland caterpillar(Gynaephora menyuanensis). Acta Prataculturae Sinica 2016; 25: 268-272.

Cao H, Zhao X, Wang S, Zhao L, Duan J, Zhang Z et al. Grazing intensifies degradation of a Tibetan Plateau alpine meadow through plant–pest interaction. Ecology & Evolution 2015; 5: 2478-2486.

Yan L. Studies of taxonomy, geographic distribution in Gynaephora genus and life-history strategies on Gynaephora menyuanensis, PhD Thesis

Xi X, Griffin JN & Sun S. Grasshoppers amensalistically suppress caterpillar performance and enhance plant biomass in an alpine meadow. Oikos 2013; 122: 1049-1057.

Carvalho MC, Queiroz PCD & Ruszczyk A. Protandry and female size-fecundity variation in the tropical butterfly Brassolis sophorae. Oecologia 1998; 116: 98-102.

Yan L, Jiang XL & Wang G. Characterization of larvae development in Gynaephora menyuanensis (Lepidoptera:Lymantriidae). Acta Pratacultural Science 2005; 90-95.

Gutiérrez S, Michalakis Y, Munster MV & Blanc S. Plant feeding by insect vectors can affect life cycle, population genetics and evolution of plant viruses. Functional Ecology 2013; 27: 610-622.

Ratte HT. Temperature and Insect Development. Environmental Physiology & Biochemistry of Insects 1985; 33-66.

Zhou HK, Zhou XM & Zhao XQ. A preliminary study of the influence of simulated greenhouse effect on a Kobresia humilis meadow. Acta Phytoecologica Sinica 2000; 24: 547- 553.

Danger M, Daufresne T, Lucas F, Pissard S & Lacroix G. Does Liebig's Law of the Minimum Scale up from Species to Communities? Oikos 2008; 117: 1741-1751.

Jankong P & Visoottiviseth P. Effects of arbuscular mycorrhizal inoculation on plants growing on arsenic contaminated soil. Chemosphere 2008; 72: 1092-1097.

Liu J, Wu L, Wei S, Xiao X, Su C, Jiang P, Song Z, Wang T & Yu Z. Effects of arbuscular mycorrhizal fungi on the growth, nutrient uptake and glycyrrhizin production of licorice (Glycyrrhiza uralensis Fisch). Plant Growth Regulation 2007; 52: 29-39.

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Copyright (c) 2018 Kelu Chen, Guoxi Shi, Wenjing Chen, Dangjun Wang, Wenying Wang, Zhen Ma, Buqing Yao, Huakun Zhou