Efficacy of Different Forms of Green Manure Crops to Reduce Verticillium dahliae in Different Soils


Brassica juncea
Soil microbial activity

How to Cite

Dalbard S, Michel VV. Efficacy of Different Forms of Green Manure Crops to Reduce Verticillium dahliae in Different Soils. Glob. J. Agric. Innov. Res. Dev [Internet]. 2021 Nov. 15 [cited 2022 May 18];8:191-205. Available from: https://www.avantipublishers.com/index.php/gjaird/article/view/1138


The efficacy of green manure crops to reduce the number of Verticillium dahliae microsclerotia in different soils was investigated. Green manures tested were Indian mustard with a high glucosinolate content and sorghum-sudangrass as biocidal plants, and Indian mustard with a low glucosinolate content and rye as non-biocidal plants. The green manure plants were applied in fresh, dried, and ensilaged form. When applied as fresh plants, the glucosinolate content determining the biocidal activity of Indian mustard was only important in loam soil but not in sandy loam soil. In the latter soil, the non-biocidal rye had significantly higher efficacy than the Indian mustard. Volatiles released by fresh and dried, but not ensilaged, Indian mustard with a high glucosinolate content strongly decreased the number of living V. dahliae microsclerotia. When the same green manure crops were added to sandy loam and clay loam soil, the effect of the high glucosinolate content Indian mustard in fresh and dried form disappeared, whereas the ensilaged green manure crops had the highest efficacy. This effect was based on the increase of the soil microbial activity and the Streptomyces population size, which were negatively correlated with the number of living V. dahliae microsclerotia in the soil.



Pegg GF, Brady BL. Verticillium wilts. CABI Publishing, Wallingford, UK. 2002. https://doi.org/10.1079/9780851995298.0000

Farr DF, Rossman AY. Fungal Databases. Systematic Mycology and Microbiology Laboratory, ARS, USDA. Retrieved 25 November 2016, from http://nt.ars-grin.gov/fungaldatabases/. 2016.

Gullino ML, Camponogara A, Gasparrini G, Rizzo V, Clini C, Garibaldi A. Replacing methyl bromide for soil disinfestation: the Italian experience and implications for other countries. Plant Dis. 2003; 97: 1012-1021. https://doi.org/10.1094/PDIS.2003.87.9.1012

Larkin RP. Green manures and plant disease management. CAB Reviews 2013; 8(037): Online publication. https://doi.org/10.1079/PAVSNNR20138037

Morris EK, Fletcher R, Veresoglou SD. Effective methods of biofumigation: a meta-analysis. Plant Soil 2020; 446: 379-392. https://doi.org/10.1007/s11104-019-04352-y

Kirkegaard J. Biofumigation for plant disease control – from the fundamentals to the farming system. In: Walters, D. (ed.). Disease control in crops: Biological and environmentally friendly approaches. Wiley-Blackwell, Oxford, UK. 2009.

de Nicola GR, Leoni O, Malaguti L, Bernardi R, Lazzeri L. A simple analytical method for dhurrin content evaluation in cyanogenic plants for their utilization in fodder and biofumigation. J. Agric. Food Chem. 2011; 59: 8065-8069. https://doi.org/10.1021/jf200754f

Garbeva P, Hol WHG, Termorshuizen AJ, KowalchukGA, de Boer W. Fungistasis and general soil biostasis – A new synthesis. Soil Biol. Biochem. 2011; 43: 469-477. https://doi.org/10.1016/j.soilbio.2010.11.020

Wiggins BE, Kinkel LL. Green manures and crop sequences influence potato diseases and pathogen inhibitory activity of indigenous Streptomycetes. Phytopathology 2005; 95: 178-185. https://doi.org/10.1094/PHYTO-95-0178

Kristensen HL, Thorup-Kristensen K. Root growth and nitrate uptake of three different catch crops in deep soil layers. Soil Sci. Soc. Am. J. 2004; 68: 529-537. https://doi.org/10.2136/sssaj2004.5290

Matthiessen JN, Shackleton MA. Biofumigation: environmental impacts on the biological activity of diverse pure and plant-derived isothiocyanates. Pest Manag. Sci. 2005; 61: 1043-1051. https://doi.org/10.1002/ps.1086

Michel VV, Mew TW. Effect of a soil amendment on the survival of Ralstonia solanacearum in different soils. Phytopathology 1998; 88: 300-305. https://doi.org/10.1094/PHYTO.1998.88.4.300

Neubauer C, Heitmann B, Müller C. Biofumigation potential of Brassiceae cultivars to Verticillium dahliae. Eur. J. Plant Pathol. 2014; 140: 341-352. https://doi.org/10.1007/s10658-014-0467-9

Harris DC, Yang JR. The relationship between the amount of Verticillium dahliae in soil and the incidence of strawberry wilt as a basis for disease risk prediction. Plant Path. 1996; 45: 106-114. https://doi.org/10.1046/j.1365-3059.1996.d01-96.x

Subbarao KV, Kabir Z, Martin FN, Koike ST. Management of soilborne disease in strawberry using vegetable rotations. Plant Dis. 2007; 91: 964-972. https://doi.org/10.1094/PDIS-91-8-0964

Falk KL, Tokuhisa JG, Gershenzon J. The effect of sulfur nutrition on plant glucosinolate content: Physiology and molecular mechanisms. Plant Biol. 2007; 9: 573-581. https://doi.org/10.1055/s-2007-965431

Butterfield EJ, DeVay JE. Reassessment of soil assays for Verticillium dahliae. Phytopathology 1977; 67: 1073-1078.

Kabir Z, Bhat RG, Subbarao KV. Comparison of media for recovery of Verticillium dahliae from soil. Plant Dis. 2004; 88: 49-55. https://doi.org/10.1094/PDIS.2004.88.1.49

Dhingra OD, Sinclair JB. Basic plant pathology methods, 2nd Ed. CRC Press, Boca Raton, FL, USA. 1995.

Schnürer J, Rosswall T. Fluorescein diacetate hydrolysis as a measure of total microbial activity. Appl. Environ. Microbiol. 1982; 43: 1256-1261. https://doi.org/10.1128/aem.43.6.1256-1261.1982

Patalano G. New practical perspectives for vegetable biocidal molecules in Italian agriculture, Bluformula brand for commercialisation of biocidal green manure and meal formulations. Agroindustria 2004; 3: 409-412.

Lazzeri L, Leoni O, Manici LM. Biocidal plant dried pellets for biofumigation. Industrial Crops Prod. 2004; 20: 59–65. https://doi.org/10.1016/j.indcrop.2003.12.018

Hawke MA, Lazarovits G. Production and manipulation of individual microsclerotia of Verticillium dahliae for use in studies of survival. Phytopathology 1994; 84: 883-890.

Michel VV, Dessimoz M, Simonnet X. First Report of Verticillium dahliae causing wilt on annual wormwood in Switzerland. Plant Dis. 2016; 100: 1235. https://doi.org/10.1094/PDIS-09-15-0971-PDN

Eash NS, Green CJ, Razvi A, Bennett WF. Soil science simplified, 5th Ed. Blackwell Publishing, Ames, IA, USA. 2008.

Mayton HS, Olivier C, Vaughn SF, Loria R. Correlation of fungicidal activity of Brassica species with allyl isothiocyanate production in macerated leaf tissue. Phytopathology 1996; 86: 267-271.

Hillel D. Soil and water: Physical principles and processes. Academic Press, New York, NY, USA. 1971.

Kirkegaard J, Sarwar M. Biofumigation potential of brassicas I. Variation in glucosinolate profiles of diverse field-grown brassicas. Plant Soil 1998; 201: 71-89. https://doi.org/10.1023/A:1004364713152

Korthals GW, Thoden TC, van den Berg W, Visser JHM. Long-term effects of eight soil health treatments to control plant-parasitic nematodes and Verticillium dahliae in agro-ecosystems. Appl. Soil Ecol. 2014; 76: 112-123. https://doi.org/10.1016/j.apsoil.2013.12.016

Ochiai N, Powelson ML, Crowe FJ, Dick RP. Green manure effects on soil quality in relation to suppression of Verticillium wilt of potatoes. Biol. Fertil. Soils 2008; 44: 1013-1023. https://doi.org/10.1007/s00374-008-0289-z

Bonanomi G, Antignani V, Capodilupo M, Scala F. Identifying the characteristics of organic soil amendments that suppress soilborne plant diseases. Soil Biol. Biochem. 2010; 42: 136-144. https://doi.org/10.1016/j.soilbio.2009.10.012

Bending GD, Lincoln SD. Inhibition of soil nitrifying bacteria communities and their activities by glucosinolate hydrolysis products. Soil Biol. Biochem. 2000; 32: 1261-1269.

Brown PD, Morra MJ. Control of soilborne plant pests using glucosinolate-containing plants. Adv. Agron. 1997; 61: 167-231. https://doi.org/10.1016/S0065-2113(08)60664-1

Friberg H, Edel-Hermann V, Faivre C, Gautheron N, Fayolle L, Faloya V, et al. Cause and duration of mustard incorporation effects on soilborne plant pathogenic fungi. Soil Biol. Biochem. 2009; 41: 2075-2084. https://doi.org/10.1016/j.soilbio.2009.07.017

Grünwald NJ, Hu S, van Bruggen AHC. Short-term cover crop decomposition inorganic and conventional soils: Characterization of soil C, N, microbial and plant pathogen dynamics. Eur. J. Plant Pathol. 2000; 106: 37-50. https://doi.org/10.1023/A:1008720731062

Davis JR, Huisman OC, Westermann DT, Hafez SL, Everson DO, Sorensen LH, et al. Effects of green manures on Verticillium wilt of potato. Phytopathology 1996; 86: 444-453.

Bubici G, Marsico AD, D’Amico M, Amenduni M, Cirulli M. Evaluation of Streptomyces spp. for the biological control of corky root of tomato and Verticillium wilt of eggplant. Appl. Soil Ecol. 2013; 72: 128-134. https://doi.org/10.1016/j.apsoil.2013.07.001

Faheem M, Raza W, Zhong W, Nan Z, Shen Q, Xu Y. Evaluation of the biocontrol potential of Streptomyces goshikiensis YCXU against Fusarium oxysporum f. sp. niveum. Biol. Control 2015; 81: 101-110. https://doi.org/10.1016/j.biocontrol.2014.11.012

Schlatter D, Fubuh A, Xiao K, Hernandez D, Hobbie S, Kinkel L. Resource amendments influence density and competitive phenotypes of Streptomyces in soil. Microb. Ecol. 2009; 57: 413-420. https://doi.org/10.1007/s00248-008-9433-4

Brabban AD, Edwards C. Characterization of growth and product formation by a thermophilic streptomycete grown in a particulate rapemeal-derived liquid medium. J. Appl. Bacteriol. 1996; 80: 651-658. https://doi.org/10.1111/j.1365-2672.1996.tb03270.x

Cohen MF, Yamasaki H, Mazzola M. Brassica napus seed meal soil amendment modifies microbial community structure, nitric oxide production and incidence of Rhizoctonia root rot. Soil Biol. Biochem. 2005; 37: 1215-1227. https://doi.org/10.1016/j.soilbio.2004.11.027

Daeschel MA, Andersson RE, Fleming HP. Microbial ecology of fermenting plant materials. FEMS Microbiol. Rev. 1987; 46: 357-367.

Yuan WM, Crawford DL. Characterization of Streptomyces lydicus WYEC108 as a potential biocontrol agent against fungal root and seed rots. Appl. Environ. Microbiol. 1995; 61: 3119-3128. https://doi.org/10.1128/aem.61.8.3119-3128.1995

Snapp SS, Date KU, Kirk W, O‘Neil K, Kremen A, Bird G. Root, shoot tissues of Brassica juncea and Cereal secale promote potato health. Plant Soil 2007; 294: 55-72. https://doi.org/10.1007/s11104-007-9228-2

Gimsing AL, Kirkegaard JA. Glucosinolates and biofumigation: fate of glucosinolates and their hydrolysis products in soil. Phytochem. Rev. 2009; 8: 299-310. https://doi.org/10.1007/s11101-008-9105-5

Warton B, Matthiessen JN, Roper MR. The soil organisms responsible for the enhanced biodegradation of metham sodium. Biol. Fertil. Soils 2001; 34: 264-269. https://doi.org/10.1007/s003740100410

Andrade OA, Mathre DE, Sands DC. Natural suppression of take-all disease of wheat in Montana soils. Plant Soil 1994; 164: 9-18. https://doi.org/10.1007/BF00010105

Latour X, Corberand T, Laguerre G, Allard F, Lemanceau P. The composition of fluorescent pseudomonad populations associated with roots is influenced by plant and soil type. Appl. Environ. Microbiol. 1996; 62: 2449-2456. https://doi.org/10.1128/aem.62.7.2449-2456.1996

Arenz BE, Bradeen JM, Otto-Hanson LK, Kinkel LL. Two grass species fail to display differing species-specific effects on soil bacterial community structures after one season of greenhouse growth. Plant Soil 2014; 385: 241-254. https://doi.org/10.1007/s11104-014-2226-2

Lauber CL, Strickland MS, Bradford MA, Fierer N. The influence of soil properties on the structure of bacterial and fungal communities across land-use types. Soil Biol. Biochem. 2008; 40: 2407-2415. https://doi.org/10.1016/j.soilbio.2008.05.021

Mazzola M, Brown J, Izzo AD, Cohen MF. Mechanism of action and efficacy of seed meal-induced pathogen suppression differ in a Brassicaceae species and time-dependent manner. Phytopathology 2007; 97: 454-460. https://doi.org/10.1094/PHYTO-97-4-0454

Kinkel LL, Schlatter DC, Bakker MG, Arenz BE. Streptomyces competition and co-evolution in relation to plant disease suppression. Res. Microbiol. 2012; 163: 490-499. https://doi.org/10.1016/j.resmic.2012.07.005

Lu P, Gilardi G, Gullino ML, Garibaldi A. Biofumigation with Brassica plants and its effect on the inoculum potential of Fusarium yellows of Brassica crops. Eur. J. Plant Pathol. 2010; 126: 387-402. https://doi.org/10.1007/s10658-009-9543-y

Larkin RP, Halloran JM. Management effects of disease-suppressive rotation crops on potato yield and soilborne disease and their economic implication in potato production. Am. J. Potato Res. 2014; 91: 429-439. https://doi.org/10.1007/s12230-014-9366-z

McGuire AM. Mustard green manures replace fumigant and improve infiltration in potato cropping system. Crop Management 2003. Online publication. https://doi.org/10.1094/CM-2003-0822-01-RS

Ramirez RA, Henderson DR, Riga E, Lacey LA, Snyder WA. Harmful effects of mustard bio-fumigants on entomopathogenic nematodes. Biol. Control 2009; 48: 147-154. https://doi.org/10.1016/j.biocontrol.2008.10.010

Brown PD, Morra MJ. Brassicaceae tissues as inhibitors of nitrification in soil. J. Agric. Food Chem. 2009; 57: 7706-7711. https://doi.org/10.1021/jf901516h

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