DNA Contents in Soil Contaminated with Heavy Metals

Authors

  • Agnieszka Wolińska The John Paul II Catholic University of Lublin, str. Konstantynów 1 I, 20-718, Lublin, Poland
  • Artur Marek Banach The John Paul II Catholic University of Lublin, str. Konstantynów 1 I, 20-718, Lublin, Poland
  • Agnieszka Kuźniar The John Paul II Catholic University of Lublin, str. Konstantynów 1 I, 20-718, Lublin, Poland
  • Zofia Stępniewska The John Paul II Catholic University of Lublin, str. Konstantynów 1 I, 20-718, Lublin, Poland
  • Marta Kłos The John Paul II Catholic University of Lublin, str. Konstantynów 1 I, 20-718, Lublin, Poland

DOI:

https://doi.org/10.15377/2410-3624.2014.01.01.4

Keywords:

Heavy metal, metal resistant bacteria, soil, t-DNA.

Abstract

The study was performed to show how industrial activity affected soil quality in terms of soil DNA quality and quantity as well as soil characteristics. Soil material originated from an urban area of the Silesia Region (SW Poland). The soil characteristics were estimated: texture, moisture, pH, redox potential (Eh), and total carbon content (TOC), followed by determination of selected heavy metals (Pb, Cd, Zn, Cr, Fe, Cu). The last step was the isolation of soil DNA, its concentration and identification of microorganisms. The results showed that although the studied soil was heavily contaminated with heavy metals, there were still some metal-resistant microorganisms able to sustain soil activity. Moreover, these organisms are not present in the NCBI database, which encourages further studies aimed at identification of new organisms that may be useful in research of metal resistance as well as soil reclamation and remediation.

Author Biographies

Agnieszka Wolińska, The John Paul II Catholic University of Lublin, str. Konstantynów 1 I, 20-718, Lublin, Poland

Institute of Biotechnology, Department of Biochemistry and Environmental Chemistry

Artur Marek Banach, The John Paul II Catholic University of Lublin, str. Konstantynów 1 I, 20-718, Lublin, Poland

Institute of Biotechnology, Department of Biochemistry and Environmental Chemistry

Agnieszka Kuźniar, The John Paul II Catholic University of Lublin, str. Konstantynów 1 I, 20-718, Lublin, Poland

Institute of Biotechnology, Department of Biochemistry and Environmental Chemistry

Zofia Stępniewska, The John Paul II Catholic University of Lublin, str. Konstantynów 1 I, 20-718, Lublin, Poland

Institute of Biotechnology, Department of Biochemistry and Environmental Chemistry

Marta Kłos, The John Paul II Catholic University of Lublin, str. Konstantynów 1 I, 20-718, Lublin, Poland

Institute of Biotechnology, Department of Biochemistry and Environmental Chemistry

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Published

2014-11-27

How to Cite

1.
Agnieszka Wolińska, Artur Marek Banach, Agnieszka Kuźniar, Zofia Stępniewska, Marta Kłos. DNA Contents in Soil Contaminated with Heavy Metals. Glob. Environ. Eng. [Internet]. 2014Nov.27 [cited 2021Sep.26];1(1):29-35. Available from: https://www.avantipublishers.com/jms/index.php/tgevnie/article/view/132

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