Anti-Pathogenic Activity of Herbs Used in Argentinean Traditional Infusion

Authors

  • I. Y. Brodkiewicz, R. D. I. Molina , G. Vega Lopez, M. R. Alberto, N. R. Vera and M. E. Arena

DOI:

https://doi.org/10.15377/2409-9813.2017.04.5

Keywords:

Traditional infusions, South American herbs, Biofilm inhibition, Anti-pathogenic activity, Lippia integrifolia.

Abstract

 The anti-pathogenic activity of Acantholippia deserticola, Haplopappus baylahuen, Lippia integrifolia and Satureja parvifolia herbs used as traditional infusion was investigated. The extracts obtained were compared with respect to chromatographic profiles and antimicrobial activities. The dichloromethane and methanol extracts of the four herbs showed significant inhibition of Staphylococcus aureus and Pseudomonas aeruginosa growth and biofilm formation. The dichloromethane extract of Lippia integrifolia that showed the highest inhibitory effect on P. aeruginosa biofilm formation, was fractionated by column chromatography using a gradient of polarity, and the activities of the fractions were evaluated. In general, the lower polar fractions inhibited biofilm in correlation with bacterial growth. However, in more polar fractions the biofilm diminution is well correlated with the inhibition of autoinducers production more than the bacterial development. The results provide scientific support for the usage of these herbs to the protection against foodborne diseases. This effect is noteworthy in L. integrifolia because the fractions showed higher growth, biofilm, and autoinducer inhibitory activity than the crude extract.

References

Bracesco N, Sánchez AG, Contreras V, Menini T and Gugliucci A. Recent advances on Ilex paraguariensis research: Minireview. J Ethnopharmacol 2011; 136: 378-384. https://doi.org/10.1016/j.jep.2010.06.032

Heck CI and de Mejia EG. Yerba Mate Tea (Ilex paraguariensis): a comprehensive review on chemistry, health implications, and technological considerations. J Food Sci 2007; 72: 138-151. https://doi.org/10.1111/j.1750-3841.2007.00535.x

Filip R, Lopez P, Giberti G, Coussio J and Ferraro G. Phenolic compounds in seven South American Ilex species. Fitoterapia 2001; 72: 774-778. https://doi.org/10.1016/S0367-326X(01)00331-8

Filip R, Ferraro GE, Bandoni AL, Bracesco N, Nunes E, Gugliucci A, et al. Mate (Ilex Paraguariensis). Recent Advances in Phytochemistry, Imperato, F., Ed.; Editorial Research Signpost: Kerala, India, 2009; pp. 113-131.

Gagliardi F. La higiene del mate, ese compa-ero lleno de bacterias. 2013 Internet: (accessed May 24, 2017).

Usach, Z. Compartir el mate aumenta el riesgo de contraer infecciones. Los Andes. 2013 (accessed May 24, 2017).

Isolabella S, Cogoi L, López P, Anesini C, Ferraro G and Filip R. Study of the bioactive compounds variation during yerba mate (Ilex paraguariensis) processing. Food Chem 2010; 122: 695-699. https://doi.org/10.1016/j.foodchem.2010.03.039

Morales G, Paredes A, Sierra P and Loyola LA. Antioxidant activity of 50% aqueous ethanol extract from Acantholippia deserticola. Biol Res 2008; 41: 151-155. https://doi.org/10.4067/S0716-97602008000200004

Vogel H, Gonzalez M, Faini F, Razmilic I, Rodríguez J, Martin JS, et al. Antioxidant properties and TLC characterization of four Chilean Haplopappus-species known as bailahuen. J Ethnopharmacol 2005; 97: 97-100. https://doi.org/10.1016/j.jep.2004.10.027

Marcial G, Sendker J, Brandt S, de Lampasona MP, Catalan CA, de Valdez GF, et al. Gastroprotection as an example: antiadhesion against Helicobacter pylori, anti-inflammatory and antioxidant activities of aqueous extracts from the aerial parts of Lippia integrifolia Hieron. J Ethnopharmacol 2014; 155: 1125-1133. https://doi.org/10.1016/j.jep.2014.06.039

Cabana R, Silva LR, Valentão P, Viturro CI and Andrade PB. Effect of different extraction methodologies on the recovery of bioactive metabolites from Satureja parvifolia (Phil.) Epling (Lamiaceae). Ind Crop Prod 2013; 48: 49-56. https://doi.org/10.1016/j.indcrop.2013.04.003

Srey S, Jahid IK and Ha SD. Biofilm formation in food industries: A food safety concern. Food Control 2013; 31; 572-585. https://doi.org/10.1016/j.foodcont.2012.12.001

Bassler BL and Losick R. Bacterially speaking. Cell 2006; 125: 237-246. https://doi.org/10.1016/j.cell.2006.04.001

Gram L, Ravn L, Rasch M, Bruhn JB, Christensen AB and Givskov M. Food spoilage interactions between food spoilage bacteria. Int J Food Microbiol 2002; 78: 79-97. https://doi.org/10.1016/S0168-1605(02)00233-7

Telford G, Wheeler D, Williams P, Tomkins PT, Appleby P, Sewell H, et al. The Pseudomonas aeruginosa quorumAnti- sensing signal molecule N-(3-oxododecanoyl)-l-homoserine lactone has immunomodulatory activity. Infect Immun 1998; 66: 36-42.

Rode TM, Langsrud S, Holck A and Moretro T. Different patterns of biofilm formation in Staphylococcus aureus under food-related stress conditions. Int J Food Microbiol 2007; 116: 372-383. https://doi.org/10.1016/j.ijfoodmicro.2007.02.017

Godin P. A new spray reagent for paper chromatography of polyols and cetoses. Nature 1954; 174: 134-134. https://doi.org/10.1038/174134a0

O'Toole GA and Kolter R. Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol 1998; 28: 449-461. https://doi.org/10.1046/j.1365-2958.1998.00797.x

Luciardi MC, Blázquez MA, Cartagena E, Bardón A and Arena ME. Mandarin essential oils inhibit quorum sensing and virulence factors of Pseudomonas aeruginosa. Food Sci Tech 2016b; 68: 373-380. https://doi.org/10.1016/j.lwt.2015.12.056

Whiteley M, Lee KM and Greenberg EP. Identification of genes controlled by quorum sensing in Pseudomonas aeruginosa. In Proceedings of the National Academy of Sciences of the United States of America, Washington, USA, 1999, 96, pp. 13904-13909. https://doi.org/10.1073/pnas.96.24.13904

Miller MB and Bassler BL. Quorum sensing in bacteria. Annu Rev Microbiol 2001; 55: 165-199. https://doi.org/10.1146/annurev.micro.55.1.165

Miller JH. Experiments in molecular genetics. Cold Spring Harbor. New York: Cold Spring Harbor Laboratory 1972.

Tura D and Robards K. Sample handling strategies for the determination of biophenols in food and plants. J Chromatogr A 2002; 975: 71-93. https://doi.org/10.1016/S0021-9673(02)00879-8

Rojo LE, Benites J, López J, Rojas M, Díaz P, Ordó-ez J, et al. Antioxidant capacity and polyphenolic content of twelve traditionally used herbal medicinal infusions from the South American Andes. BLACPMA 2009; 8: 498-508.

Torres-Carro R, Isla MI, Ríos J.L, Giner RM and Alberto MR. Anti-inflammatory properties of hydroalcoholic extracts of Argentine Puna plants. Food Res Int 2015; 67: 230-237 https://doi.org/10.1016/j.foodres.2014.11.012

Silva M and Sammes PG. A new diterpenic acid and other constituent of Haplopappus foliosus and H. Angustifolius. Phytochemistry 1973; 12: 1755-1758. https://doi.org/10.1016/0031-9422(73)80397-8

Chiang MT, Bittner M, Silva M, Mondaca A, Zemelman R and Sammes PG. A prenylated coumarin with antimicrobial activity from Haplopappus multifolius. Phytochemistry 1982; 21: 2753-2755. https://doi.org/10.1016/0031-9422(82)83119-1

Urzúa A, Torres R, Mu-oz M and Palacios Y. Comparative antimicrobial study of the resinous exudates of some Chilean Haplopappus (Asteraceae). J Ethnopharmacol 1995; 45: 71- 74. https://doi.org/10.1016/0378-8741(94)01196-7

Pascual ME, Slowing K, Carretero E, Sánchez Mata D and Villar A. Lippia: traditional uses, chemistry and pharmacology: a review. J Ethnopharmacol 2001; 76: 201- 214. https://doi.org/10.1016/S0378-8741(01)00234-3

Kondo Y, Takano F and Hojo H. Suppression of chemically and immunologically induced hepatic injuries by gentiopicroside in mice. Planta Med 1994; 60: 414-416. https://doi.org/10.1055/s-2006-959521

Liu J, Liu Y and Klaassen CD. (1994). The effect of Chinese hepatoprotective medicines on experimental liver injury in mice. J Ethnopharmacol 1994; 42: 183-191. https://doi.org/10.1016/0378-8741(94)90084-1

Muschietti L, Van Baren C, Coussio J, Vila R, Clos M, Caigueral S, et al. Chemical composition of the leaf oil of Satureja odora and Satureja parvifolia. J Essent Oil Res 1996; 8: 681-684. https://doi.org/10.1080/10412905.1996.9701042

Viturro CI, Molina A, Guy I, Charles B, Guinaudeau H and Fournet A. Essential oils of Satureja boliviana and Satureja parvifolia growing in the region of Jujuy, Argentina. Flavour Fragr J 2000; 15: 377-382. https://doi.org/10.1002/1099-1026(200011/12)15:6<377::AIDFFJ926>3.0.CO;2-P

Feresin GE, Tapia A, López SN and Zacchino SA. Antimicrobial activity of plants used in traditional medicine of San Juan province, Argentine. J Ethnopharmacol 2001; 78: 103-107. https://doi.org/10.1016/S0378-8741(01)00322-1

Hernández NE, Tereschuk ML and Abdala LR. Antimicrobial activity of flavonoids in medicinal plants from Tafí del Valle (Tucuman, Argentina). J Ethnopharmacol 2000; 73: 317-322. https://doi.org/10.1016/S0378-8741(00)00295-6

Muschietti L, Derita M, Sulsen V, de Dios Munoz J, Ferraro G, Zacchino S, et al. In vitro antifungal assay of traditional Argentine medicinal plants. J Ethnopharmacol 2005; 102: 233-238. https://doi.org/10.1016/j.jep.2005.06.017

Lemos TLG, Matos FJA, Alencar JW, Craveiro AA, Clark AM and Mc Chesney JD. Antimicrobial activity of essential oils of Brazilian plants. Phytother Res 1990; 4: 82-84. https://doi.org/10.1002/ptr.2650040210

Otto M. Quorum-sensing control in Staphylococci a target for antimicrobial drug therapy? FEMS Microbiol Lett 2004; 241: 135-141. https://doi.org/10.1016/j.femsle.2004.11.016

Rice SA, McDougald D, Kumar N and Kjelleberg S. The use of quorum-sensing blockers as therapeutic agents for the control of biofilm-associated infections. Curr Opin Invest Dr 2005; 6: 178-184.

Gao M, Teplitski M, Robinson JB and Bauer WD. Production of substances by Medicago truncatula that affect bacterial quorum sensing. Mol Plant Microbe Interact 2003; 16: 827- 834. https://doi.org/10.1094/MPMI.2003.16.9.827

Gilabert M, Ramos AN, Schiavone MM, Arena ME and Bardon A. Bioactive sesqui- and diterpenoids from the Argentine liverwort Porella chilensis. J Nat Prod 2011; 74: 574-579. https://doi.org/10.1021/np100472d

Teplitski M, Robinson JB and Bauer WD. Plants secrete substances that mimic bacterial N-acyl homoserine lactone signal activities and affect population density-dependent behaviors in associated bacteria. Mol Plant Microbe Interact 2000; 13: 637-648. https://doi.org/10.1094/MPMI.2000.13.6.637

Amaya S, Pereira JA, Borkosky SA, Valdez JC, Bardón A and Arena ME. Inhibition of quorum sensing in Pseudomonas aeruginosa by sesquiterpene lactones. Phytomedicine 2012; 19: 1173-1177. https://doi.org/10.1016/j.phymed.2012.07.003

Gilabert M, Cartagena E, Escobar G, Bardón A and Arena ME. Volatile terpenoids from water Pepper (Polygonum punctatum) against Pseudomonas aeruginosa and Staphylococcus aureus virulence strategies. Glob J Agric Innov Res Dev 2014; 1: 3-10. https://doi.org/10.15377/2409-9813.2014.01.01.1

Socolsky C, Arena ME, Asakawa Y and Bardon A. Antibacterial prenylated acylphloroglucinols from the fern Elaphoglossum yungense. J Nat Prod 2010; 73: 1751-1755. https://doi.org/10.1021/np1001779

Luciardi MC, Pérez Hernández MV, Muruaga N, Bardón A, Arena ME and Cartagena E. Volatiles from subtropical Convolvulaceae that interfere with bacterial cell-to-cell communication as potential antipathogenic drugs. Evid- Based Compl Alt 2016a. (accessed May 24, 2017).

Al-Nahari AAM, Almasaudi SB, El-Ghany EMA, Barbour E, Al Jaouni SK and Harakeh S. Antimicrobial activities of Saudi honey against Pseudomonas aeruginosa. Saudi J. Biol Sci 2015; 22: 521-525. https://doi.org/10.1016/j.sjbs.2015.04.006

Nuño G, Alberto MR, Arena ME, Zampini C and Isla MI. Effect of Zuccagnia punctata Cav. (Fabaceae) extract on proinflammatory enzymes and on planktonic cells and biofilm from Staphylococcus aureus. Toxicity studies. Saudi J Biol Sci 2016.

Downloads

Published

2018-01-29

How to Cite

1.
I. Y. Brodkiewicz, R. D. I. Molina , G. Vega Lopez, M. R. Alberto, N. R. Vera and M. E. Arena. Anti-Pathogenic Activity of Herbs Used in Argentinean Traditional Infusion. Glob. J. Agric. Innov. Res. Dev [Internet]. 2018Jan.29 [cited 2021Jun.17];4(1):36-4. Available from: https://www.avantipublishers.com/jms/index.php/gjaird/article/view/710

Issue

Section

Articles